[摘要] 目的 研究葶藶生脈方中黃芪、麥冬皂苷的含量測定方法，并以其含量為指標優(yōu)選黃芪、麥冬的最佳提取工藝。 方法 選用以香草醛-冰醋酸-高氯酸為顯色劑，紫外分光光度法測定總皂苷的含量。采用正交試驗法，以總皂苷的含量為評價指標，考察乙醇濃度、料液比和提取時間對提取工藝的影響，優(yōu)選黃芪、麥冬的最佳提取工藝。 結果 本研究中黃芪甲苷在8.88～44.40 μg范圍內呈良好的線性關系，精密度、穩(wěn)定性、重現(xiàn)性試驗的RSD值均≤1.19%，平均加樣回收率為101.78%，RSD值為1.85%（n = 9）。最佳提取工藝：8倍量80%的乙醇回流提取2次，每次2 h。 結論 該方法操作簡便，準確、重現(xiàn)性好，可作為葶藶生脈方中黃芪、麥冬皂苷質量控制的方法。
　　[關鍵詞] 黃芪；麥冬；總皂苷；葶藶生脈方；提取工藝；正交試驗
　　[中圖分類號] R284.2 [文獻標識碼] A [文章編號] 1673-7210（2018）02（c）-0128-04
　　Extraction and content determination of total saponins from Astragali Radix and Ophiopogonis Radix in Tingli Shengmai Decoction
　　LI Jiajia1 ZHENG Wenli2 ZHANG Huatan3 LI Chunhua4
　　1.Grduate School， Hebei University of Chinese Medicine， Hebei Province， Shijiazhuang 050090， China； 2.Department of Pharmacy， Shijiazhuang Second Hospital， Hebei Province， Shijiazhuang 050051， China； 3.Shijiazhuang Keren Pharmaceutical Technology Co.， Ltd， Hebei Province， Shijiazhuang 050035， China； 4.College of Pharmacy， Hebei University of Chinese Medicine， Hebei Province， Shijiazhuang 050200， China
　　[Abstract] Objective To study the content determination method of total saponins from Astragali Radix and Ophiopogonis Radix in Tingli Shengmai Decoction， and to optimize the optimum extraction process of Astragali Radix and Ophiopogonis Radix taking the contents as indices. Methods The vanillic aldehyde-glacial acetic acid-perchloric acid was selected as color developing agent， and the contents of total saponins were detected by ultraviolet spectrophotometry. By using orthogonal experiment， taking the contents of total saponins as indices， the effects of ethanol concentration， solid-liquid ratio and extraction duration for the extraction process were investigated， so as to optimize the optimum extraction process of Astragali Radix and Ophiopogonis Radix. Results In this study， astragaloside Ⅳ showed a good linear relationship within the range of 8.88-44.40 μg， all the RSD values of degree of precision， stability and repeatability experiments were ≤1.19%， the average recovery rate was 101.78%， RSD was 1.85% （n = 9）. The optimum extraction process： reflux extraction of 8 times amount of 80% ethanol was taken for 2 times， once 2 h. Conclusion The method is simple， accurate and reproducible， which can be used as a method for the quality control of Astragali Radix and Ophiopogonis Radix in Tingli Shengmai Decoction
　　[Key words] Astragali Radix； Ophiopogonis Radix； Total saponins； Tingli Shengmai Decoction； Extraction process； Orthogonal experiment

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