东方亚洲欧a∨人在线观看|欧美亚洲日韩在线播放|日韩欧美精品一区|久久97AV综合

        不同雷公藤制劑中6個(gè)成分的含量研究

        發(fā)布時(shí)間:2018-06-23 來(lái)源: 散文精選 點(diǎn)擊:

        http://img1.qikan.com.cn/qkimages/yxxi/yxxi201805/yxxi20180523-1-l.jpghttp://img1.qikan.com.cn/qkimages/yxxi/yxxi201805/yxxi20180523-2-l.jpghttp://img1.qikan.com.cn/qkimages/yxxi/yxxi201805/yxxi20180523-3-l.jpghttp://img1.qikan.com.cn/qkimages/yxxi/yxxi201805/yxxi20180523-4-l.jpg
          摘   要:目的  建立測(cè)定雷公藤制劑中的6種成分(雷公藤甲素、雷公藤內(nèi)酯酮、雷酚內(nèi)酯、雷公藤次堿、雷公藤紅素及雷公藤內(nèi)酯甲)的高效液相色譜方法。方法  采用Agilent TC-C18柱(250 mm×4.6 mm,5 μm),以10 mmol/L乙酸銨水溶液-乙腈為流動(dòng)相梯度洗脫,流速為0.75 ml/min,檢測(cè)波長(zhǎng)為225 nm,柱溫為25 ℃。結(jié)果  6種成分的進(jìn)樣濃度為0.1~156.25 μg/ml范圍內(nèi)與峰面積積分值線性關(guān)系良好,平均回收率為98.5%(RSD=1.9%)、99.2%(RSD=1.9%)、99.5%(RSD=1.5%)、98.7%(RSD=1.2%)、98.8%(RSD=1.6%)、99.0%(RSD=1.7%)。結(jié)論  所建立的測(cè)定方法操作方便,重復(fù)性強(qiáng),結(jié)果準(zhǔn)確,可用于雷公藤及其相關(guān)產(chǎn)品的質(zhì)量分析;不同雷公藤制劑中6個(gè)成分的含量存在差異。
          關(guān)鍵詞:雷公藤制劑;雷公藤化學(xué)成分;反相高效液相色譜法
          中圖分類(lèi)號(hào):R284.1                                   文獻(xiàn)標(biāo)識(shí)碼:A                               DOI:10.3969/j.issn.1006-1959.2018.05.023
          文章編號(hào):1006-1959(2018)05-0070-04
          Study on the Content of Six Components in Different Tripterygium Wilfordii Preparations
          QI Ai-rong1,F(xiàn)U Bo1,LIU Xiao-yan1,LI Shun-min1,WEN Shan-shi2,ZHENG Ping3,
          CHEN Jian-ping3
          (1.4th College of Clinical Medicine,Guangzhou University of Traditional Chinese Medicine,Shenzhen 518033,Guangdong,China;
          2.Nanao People"s Hospital,Dapeng New District,Shenzhen 518121,Guangdong,China;
          3.Key Laboratory of Traditional Chinese Medicine Preparations,Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 518033,Guangdong,China)
          Abstract:Objective  To establish a high performance liquid chromatography method for the determination of 6 components in Tripterygium wilfordii(Triptolide,triptolide,lycopolide,Tripterygium,tripterin,and triptolide a).Methods  A Agilent TC-C18 column of (250 mm×4.6 mm,5 μm)was used.The mobile phase gradient elution was 10 mmol/L ammonium acetate aqueous solution and acetonitrile.The flow rate was 0.75 ml/min,the detection wavelength was 225 nm,and the column temperature was 25 ℃.Results  The injection concentration of the six components was 0.1~156.25 μg/ml and the peak area integral value was linear,the average recovery rate was 98.5%(RSD=1.9%),99.2%(RSD=1.9%),99.5%(RSD=1.5%),98.7%(RSD=1.2%),98.8%(RSD=1.6%),99.0%(RSD=1.7%).Conclusion  The established method is convenient, reproducible and accurate.It can be used for the quality analysis of Tripterygium wilfordii and its related products,and the content of 6 components in different Tripterygium wilfordii preparations is different.
          Key words:Tripterygium wilfordii preparation;Chemical constituents of Tripterygium wilfordii;Reversed phase high performance liquid chromatography

        相關(guān)熱詞搜索:制劑 含量 成分 研究 雷公藤

        版權(quán)所有 蒲公英文摘 smilezhuce.com